Commentaries

• director Sam Raimi and cast members Tobey Maguire, Kirsten Dunst, James Franco, Thomas Haden Church, Topher Grace, and Bryce Dallas Howard
• producers Laura Ziskin, Avi Arad and Grant Curtis, editor Bob Murawski and special effects supervisor Scott Stokdyk

Featurettes

• Covered in Black: Creating Venom - Follows the cinematic creation of Spider-Man's archrival
• Fighting, Flying & Driving: The Stunts - Wherein Stunt Coordinator/2nd Unit Director Dan Bradley and Stunt Coordinator Scott Rogers provide a behind-the-scenes peek at the various "gags" in the film
• Grains of Sand: Building Sandman - Details the challenges Sony Pictures Imageworks faced in creating the iconic villain
• Hanging On: Gwen Stacy and the Collapsing Floor - Where stunt coordinator Scott Rogers explores the logistics of the dangerous stunt performed by Bryce Dallas Howard
• Inside The Editing Room
• On Location Cleveland - The Chase on Euclid Avenue
• On Location New York - From Rooftops to Backstreets
• On The Set: Spider-Man 3 - Target Bonus DVD
• Re-Imagining the Goblin - plores the changes in the New Goblin character from page to screen
• Tangled Web: The Love Triangles of Spider-Man 3
• The Science of Sound
• Wall of Water - where the viewer is taken into the creation of one of the biggest stunts in the film

Other

• Bloopers
• English & Spanish Subtitles
• Photo Galleries
• Production Notes Booklet
• Snow Patrol Music Video
• The 5 Winning Webisodes - Target Bonus DVD
• The Spider-Man 3 Spin Your Own Webisode Contest - Target Bonus DVD
• The Winners at the Award Ceremony held at the LA Film Festival - Target Bonus DVD
• Theatrical TV Spots From Around the World

• Audio Commentary
• Outtakes
• Featurettes